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发布于:2018-9-21 20:33:58  访问:0 次 回复:0 篇
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The Most Up-To-Date Rapamycin Is Twice The Enjoyable
?ivanovii DNA equivalent to approx. to 1?��?106, 1?��?105, 1?��?104, 1?��?103, 100, 10 and 1 genome equivalents per reaction. This experimental approach was followed including or not including the IAC in each reaction, to determine whether the simultaneous coamplification of the IAC could affect the detection limit of the assay. Table?2 shows the mean CT values of a total of 10 PCR replicates (20 replicates for 10, and 1 genome equivalents) from two independent experiments. Positive amplifications in all the replicates were obtained when the concentration of the target in the PCR was 10 genome equivalents or more and 1 genomic equivalent was detected with a 45 Rapamycin in vivo and 30% probability, in the absence or presence of IAC, respectively. This difference in the probability of detection was analysed statistically, and no significant differences (P?Bleomycin of the PCR to duplicate the amplicon molecules in each cycle and is calculated from the slope of the linear regression curve (s) from the equation E?=?10?1/s�C 1. The smcL and smcL-IAC linear regression curves were constructed by plotting the mean CT values obtained for each dilution against the logarithms of the number of genome equivalents per reaction. The calculated R2 values (smcL, 0��9989 and smcL-IAC, 0��9995) were MG-132 cost close to 1, showing a high linearity over a dynamic quantification range of at least 6 logs (from 1?��?106 to 10 genome equivalents). The slope values (smcL, ?3��2258 and smcL-IAC, ?3��3609) correspond to E values of 1��042 and 0��984 for smcL and smcL-IAC, respectively. They were very close to the theoretical values (s?=??3��3219 and E?=?1) showing an excellent efficiency. The LOQ was established as the lowest sample dilution in which the 99% confidence interval does not overlap with that of the next dilution. A statistical analysis (anova and Tukey post-hoc method, ��?=?0��01) determined the absence of overlapping CT values, and therefore confirmed reliable L.?ivanovii quantification was possible down to 10 genome equivalents of this bacterium. The smcL-IAC RTi-PCR assay was used for the identification and quantification of L.?ivanovii in three different artificially contaminated biological matrices; i.e. raw sheep milk, blood and amniotic fluid. The RTi-PCR assays yielded similar results in the different matrices in terms of absolute detection values (Tables?3, 4 and 5).
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